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mcf12a human normal breast cell line  (Marine Biological Laboratory)

 
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    Marine Biological Laboratory mcf12a human normal breast cell line
    Mcf12a Human Normal Breast Cell Line, supplied by Marine Biological Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcf12a+human+normal+breast+cell+line/mcf12a+human+normal+breast+cell+line/pmc03679486-262-0-13
    Average 90 stars, based on 1 article reviews
    mcf12a human normal breast cell line - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Negative regulation of p53 by Ras superfamily protein RBEL1A
    Article Snippet: Cell culture, transfection and RBEL1A knockdown Cell lines MCF-7 (breast cancer), RKO (colon cancer), H1299 (lung cancer), HEK293T (human embryonic kidney cells) and p53 −/− / MDM2 −/− mouse embryonic fibroblasts (kindly provided by Dr Guillermina Lozano, U. T. M. D. Anderson Cancer Center, USA) were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (Gemini Bio-Products Inc., West Sacramento, CA, USA). .. MCF12A human normal breast cell line (kindly provided by Dr G. Wayne Zhou, Marine Biological Lab, USA) was maintained in DMEM supplemented with 10% FBS and 10 μg/ml insulin, 20 ng/ml hEGF, 100 ng/ml cholera toxin, 500 ng/ml hydrocortisone, 10 IU/ml penicillin–streptomycin (Mediatech, Inc., Manassas, VA, USA) and 200 μM L-glutamine (Mediatech, Inc.). pCEP4-RBEL1A (WT, T57N, 1–235)-expressing cells (pooled clones) or vector-alone-expressing cells (pooled clones) were generated by stable transfection. .. All transfections were done using either Lipofectamine 2000 (Invitrogen/Life Technologies, Grand Island, NY, USA) or TransIT-LT1 (Mirus, Madison, WI, USA).

    Stable Transfection:

    Article Title: Negative regulation of p53 by Ras superfamily protein RBEL1A
    Article Snippet: Cell culture, transfection and RBEL1A knockdown Cell lines MCF-7 (breast cancer), RKO (colon cancer), H1299 (lung cancer), HEK293T (human embryonic kidney cells) and p53 −/− / MDM2 −/− mouse embryonic fibroblasts (kindly provided by Dr Guillermina Lozano, U. T. M. D. Anderson Cancer Center, USA) were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (Gemini Bio-Products Inc., West Sacramento, CA, USA). .. MCF12A human normal breast cell line (kindly provided by Dr G. Wayne Zhou, Marine Biological Lab, USA) was maintained in DMEM supplemented with 10% FBS and 10 μg/ml insulin, 20 ng/ml hEGF, 100 ng/ml cholera toxin, 500 ng/ml hydrocortisone, 10 IU/ml penicillin–streptomycin (Mediatech, Inc., Manassas, VA, USA) and 200 μM L-glutamine (Mediatech, Inc.). pCEP4-RBEL1A (WT, T57N, 1–235)-expressing cells (pooled clones) or vector-alone-expressing cells (pooled clones) were generated by stable transfection. .. All transfections were done using either Lipofectamine 2000 (Invitrogen/Life Technologies, Grand Island, NY, USA) or TransIT-LT1 (Mirus, Madison, WI, USA).



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